The 180km bike course consists of two sections: a 2-loop course in the SCTEX and a 6-loop course in Subic Bay.

 

The first section begins from Rizal Avenue, where participants turn right onto SFEX (Subic Freeport Expressway) road, proceeding towards Tipo gate to enter the SCTEX (Subic Clark Tarlac Expressway). Riders continue towards Dinalupihan, and Florida Blanca reaching the first turnaround at KM 29, then back towards the second turnaround just before Tipo Gate at KM 45, completing the first loop. They then head to the third turnaround at KM 62, returning to Tipo gate at KM 80, exiting SCTEX and returning to Subic via SFEX.
 

Exiting Rizal Avenue, heading to Argonaut marks the start of the second section, a 6-loop course. From Rizal Avenue, participants turn left onto Argonaut Ave, then right onto NCT (New Container Terminal) road, completing a loop and exiting onto San Bernardo road, then back to Argonaut Ave. Participants repeat this loop six times, passing through NCT and San Bernardo roads, before returning to Argonaut for the first turnaround at KM 105. They then proceed to complete five more loops, with turnarounds at KM 119.5, KM 134, KM 148.5, and KM 163. After the final loop at KM 177.5, they head to Malawaan Park, exit to Rizal Avenue, turn right towards SBECC (Subic Bay Convention Center) to conclude the bike course and enter transition 2.